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Fig. S1 Midbrain vasculature at 1.5 dpf. (A) Projected confocal image of a 1.5-dpf Tg(kdrl:eGFP) larva showing that some angiogenic sprouts (green arrows) were observed in the midbrain. Inset 1, filopodium-like sprout; Insets 2 and 3, sprouts with an expanded tip. Red and green arrows point to sprouts originated from the choroidal vascular plexus (CVP) or midbrain vasculature, respectively. The dashed square delineates the midbrain position. Scale, 50 μm. (B) 3-D reconstruction of the midbrain vasculature shown in (A). Yellow, basal communicating artery (BCA); white, midbrain vessels; blue, CVP.
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