ZFIN Image: Liu et al., 2012, Fig. S3

Image

Figure Caption

Fig. S3 Morpholino efficiency test and hemorrhage phenotype for integrin morphants.

(A) itgav^MOf and itgav^MOr primers were used to amplify integrin α v around the exonintron boundary targeted by the itgav^e3i3 MO. Black arrows denote the wildtype band. Stars denote misspliced new products seen only in the morphants. (B) Hemorrhages (arrow) are observed in the head of itgav^ATG morphants between 50 – 52 hpf. (C) itgb8^MOf and itgb8^MOr primers were used to amplify integrin β 8 around the exonintron boundary targeted by both itgb8^e2i2 and itgb8e²ⁱ¹ morpholinos. Black arrows denote the wildtype band, while a star denotes misspliced new products seen only in the morphants. (D) Hemorrhages (arrow) are observed in the head of itgb8^e2i1 morphants.

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 363(1), Liu, J., Zeng, L., Kennedy, R.M., Gruenig, N.M., and Childs, S.J., betaPix plays a dual role in cerebral vascular stability and angiogenesis, and interacts with integrin alphavbeta8, 95-105, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.