ZFIN Image: Izumi et al., 2012, Fig. 5

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Figure Caption

Fig. 5 Impaired ISV growth after knockdown of zebrafish fbxw7 expression.

Following injection with control morpholino (control MO) or fbxw7 transcription blocking morpholino (fbxw7 ATG MO), the fluorescent vasculature of Tg(kdrl:EGFP)^s843 transgenic embryos was examined by live microscopy. Outgrowth of ISVs from the dorsal aorta (DA) and formation of the DLAV (as indicated in the left image) were impaired in fbxw7 morphant embryos (arrowheads) at 32 hpf (A). Scale bar is 70 μm. Protein extracts from control and fbxw7 ATG MO embryos at 24 hpf and 32 hpf were analyzed by Western blotting (B). Note transient reduction of the Fbxw7 band (asterisk). Tubulin was used as loading control. Quantification of the number of ECs per ISV in control MO and fbxw7 ATG MO injected embryos at 32 hpf (based on 3 independent experiments) (C). Proliferation of endothelial cells at 32 hpf in ISVs of Tg(fli1a:nEGFP)^y7 x Tg(kdrl:HsHRAS-mCherry)^s896 double transgenic embryos injected with control MO or Fbxw7 ATG MO (D). Error bars indicate SEM. P value (*) in (C) is <0.05.

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Acknowledgments

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