|
Fig. 2
The effects of X-13 on Human Umbilical Vein Endothelial Cell (HUVEC) invasion, migration and tube formation. HUVEC cultures were incubated with X-13 in the presence or absence of inhibitors for different periods in different assays and VEGF-treated cell cultures served as a positive control. Representative images show that X-13 induced HUVEC migration (A), invasion (B) and tube formation (C) in HUVEC cultures. L-NAME and LY294002 significantly inhibited X-13-induced cell invasion (A), migration (B) and tube formation (C). The bar chart shows quantitative data. Data are expressed as the mean ± SD. Results were obtained from four independent experiments (* X-13 vs. vehicle control group, p < 0.05; # LY294002 plus X-13 vs. X-13, p < 0.05; ☆ L-NAME plus X-13 vs. X-13, p < 0.05).