IMAGE

Fig. 5

ID
ZDB-IMAGE-120710-12
Source
Figures for Soares et al., 2012
Image
Figure Caption

Fig. 5 GFP rescue sensor assay.

A) Single cell embryos were injected with the GFP reporter (50 ng/μL) in the presence or absence of MOmiR-2188, miR-2188 and scrambled duplexes or a mixture of both MOmiR-2188 and miR-2188 duplex. Fluorescence levels were observed at 24 hpf and representative embryos of each condition were photographed. Fluorescence was recovered in MOmiR-2188+ miR-2188 injected embryos, when compared to miR-2188 duplex injected embryos. B) Embryo lysates were prepared from 24 hpf embryos injected with the GFP reporter in the presence or absence of MOmiR-2188, miR-2188 and scrambled duplexes or a mixture of MOmiR-2188 and miR-2188. Protein levels were determined by western blot analysis and β-tubulin was used as an internal control. C) Quantification of the reporter proteins (%). Asterisks indicate conditions where GFP expression was significantly down regulated by miR-2188 relative to control conditions. miR-2188 injected embryos showed a statistically significant down regulation of GFP levels, indicating that Nrp2a 3′UTR is a true miR-2188 target. Co-injection of the MOmiR-2188 with the miR-2188 duplex resulted in the rescue of GFP fluorescence, further confirming that Nrp2a is a bona fide target of miR-2188. Data are mean +/stdev, p<0,005 (t test, unpaired), n>3. All lanes were normalized to the β-tubulin signal.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One