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Fig. 4 Neriifolin-induced DA neuronal death is apoptotic and requires p53.
(A?D′ ′′) Low (A?D) and high (A′?D′ ′′) magnification views of VFB DA neurons in control (A-A′ ′′) vs Neriifolin-treated embryos (B-B′ ′′), and sympathetic (Sym) NA neurons in control (C-C′ ′′) vs Neriifolin-treated embryos (D-D′ ′′). Ventral views of 60 hpf embryos were shown. Neriifolin treatment was carried out from 24 hpf to 60 hpf. (E) Injection of the p53-MO into embryos at one-cell stage protected DA neurons from cell death induced by Neriifolin. Data are the averages ± SEM from 6 embryos in a single experiment that was repeated twice with similar results.