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Figure Caption
Fig. 2 Human atp1a3 rescues DA neurons in Neriifolin-treated embryos.
(A) The expression pattern of atp1a3a in wild-type embryos at 48 hpf. (B) The schematic diagram of the plasmid constructs used for the rescue experiments in zebrafish embryos. (C) RT-PCR detection of the expression of human atp1a3 in zebrafish embryos after injection and heat shock. (D?E) Quantification (D) and representative images (E) of VFB DA neurons in 5 μM Neriifolin-treated embryos that express either GFP or human atp1a3. Data are the averages ± SEM from 9 embryos in a single experiment that was repeated twice with similar results.
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