ZFIN Image: Jao et al., 2012, Fig. S8

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Figure Caption

Fig. S8

Most gfap:GFP⁺ radial glia are Sox2⁺ neural precursors, which are located close to the apical surface and are mutually exclusive with motoneurons. (A-D) Transverse cryosections through the spinal cord of wild-type embryos harboring the gfap:GFP transgene were stained with anti-Sox2 antibody at 24 hpf. Most, if not all, gfap:GFP⁺ cells (A) are also Sox2⁺ (B, merged channels in C,D). (E-P) Transverse cryosections through the spinal cord of gle1 morphants harboring the mnx1:TagRFP-T transgene were stained with anti-Sox2 antibody at 36 hpf. Note that a portion of mnx1:TagRFP-T⁺ motoneurons (arrowheads) are located in close proximity to some of the Sox2⁺ neural precursors (arrows). However, these two signals do not overlap (merged channels in G,K,O and H,L,P). Nuclei were counterstained by TO-PRO-3. Scale bars: 7.5 μm in D; 10 μm in H,L,P.

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Expression / Labeling details

Acknowledgments

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