ZFIN Image: Jao et al., 2012, Fig. S6

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Figure Caption

Fig. S6

TUNEL assays rarely detect motoneuron death in Gle1-deficient embryos. (A-L) Transverse cryosections through the spinal cords of the control (A-D), gle1^-/- mutant (E-H) and gle1 morphant (I-L) embryos with their motoneuron nuclei labeled by mnx1:H2A-TagRFP-T transgene at 49 hpf. Sections were subjected to TUNEL reactions to reveal apoptotic cells. Nuclei were counterstained by TO-PRO-3. Note that motoneurons (H2A-TagRFP-T⁺) are rarely labeled by TUNEL (<1%, examples indicated by arrows) in the gle1^-/- mutant (E-H) and gle1 morphant (I-L) embryos, despite the fact that TUNEL⁺ signals are evident in those sections with deficient Gle1 activity (E-L). Scale bar: 10 μm.

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