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Fig. 1
Workflow diagram.
Embryos were either uninjected or injected with etsrp RNA at the one cell stage, then raised until the late gastrulation stages when the flk1-gfp transgenic reporter is induced ectopically. Equal paired groups of embryos were pooled for mRNA extraction, library construction, and solexa mRNA-sequencing. Samples were pre-validated prior to sequencing by RT-PCR with fli1A, the 32UTR of etsrp, as well as scl, which were all preferentially induced as expected by etsrp overexpression (e) relative to uninjected controls (u).
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