PUBLICATION

Identification of Vascular and Hematopoietic Genes Downstream of etsrp by Deep Sequencing in Zebrafish

Authors
Gomez, G., Lee, J.H., Veldman, M.B., Lu, J., Xiao, X., and Lin, S.
ID
ZDB-PUB-120327-3
Date
2012
Source
PLoS One   7(3): e31658 (Journal)
Registered Authors
Gomez, Gustavo, Lin, Shuo
Keywords
none
MeSH Terms
  • Hematopoiesis/genetics*
  • Gene Expression Profiling
  • RNA/genetics
  • Animals
  • Oligonucleotide Array Sequence Analysis
  • Mice
  • Blood Vessels/embryology*
  • High-Throughput Nucleotide Sequencing
  • Zebrafish Proteins/deficiency
  • Zebrafish Proteins/genetics*
  • Base Sequence
  • Gene Expression Regulation, Developmental
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Animals, Genetically Modified
(all 15)
PubMed
22438865 Full text @ PLoS One
Abstract

The transcription factor etsrp/Er71/Etv2 is a master control gene for vasculogenesis in all species studied to date. It is also required for hematopoiesis in zebrafish and mice. Several novel genes expressed in vasculature have been identified through transcriptional profiling of zebrafish embryos overexpressing etsrp by microarrays. Here we re-examined this transcriptional profile by Illumina RNA-sequencing technology, revealing a substantially increased number of candidate genes regulated by etsrp. Expression studies of 50 selected candidate genes from this dataset resulted in the identification of 39 new genes that are expressed in vascular cells. Regulation of these genes by etsrp was confirmed by their ectopic induction in etsrp overexpressing and decreased expression in etsrp deficient embryos. Our studies demonstrate the effectiveness of the RNA-sequencing technology to identify biologically relevant genes in zebrfish and produced a comprehensive profile of genes previously unexplored in vascular endothelial cell biology.

Genes / Markers
Figures
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
zn1TgTransgenic Insertion
    1 - 1 of 1
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    No data available
    Fish
    Fish
    zn1Tg
    1 - 1 of 1
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    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    Marker Marker Type Name
    grcfpEFGgrcfp
    1 - 1 of 1
    Show
    Mapping