ZFIN Image: Gomez et al., 2012, Fig. 2

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Figure Caption

Fig. 2

Verification of RNA-seq data.

Wild type and etsrp injected, etsrp oe, embryos were collected at late stages of gastrulation and processed by WISH. Ectopic induction was detected as random positively labeled cells in embryos injected with etsrp RNA at the one cell stage. Endogenous expression was observed in the presumptive forerunner cells (arrows) of wild-type uninjected embryos in (C) she and (E) spa17. Endogenous expression was also observed in (K) tmem119b (L) myl9 (AD2) sh3bp4 (AN2) hmha1 (AT2) aff3 and (AW2) acsbg2. Ectopic induction is clearly detected in 49 of the 50 genes examined, but not (AT2) aff3, which has a relatively high level of endogenous expression at this stage. Wild type uninjected embryos are positioned on the left column with their etsrp oe counterparts on the right for each gene, anterior is facing left where possible. Scale bar: 250 μm.

Acknowledgments

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