Fig. S6 Confocal imaging of live appa+/is22Gt; Tg(flk1:moesin1-egfp)+/is1 embryo before completion of venous vessel formation. A–D: Lateral view, 24 hours post fertilization (hpf). A: Appa-red fluorescent protein (RFP) in the head is enriched in the eye surrounding the lens (yellow arrows). Appa-RFP has not accumulated at the veins in the head. C,D: Appa-RFP accumulates at the somite boundaries in the trunk (yellow arrow) but is absent from the posterior caudal vein region (white arrow). E–H: Lateral view, 28 hpf. E,F: Appa-RFP does not show enrichment at the veins in the head at this time. G,H: Accumulation of Appa-RFP at the somite boundaries has increased (yellow arrows) and is detected in the region of the posterior caudal vein (white arrows). Scale bars = 50 μm.
                
                    
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                        Acknowledgments
                    
                    
                
                
            
        
        
    
        
            
            
                
                    
                        
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