ZFIN Image: Cast et al., 2012, Fig. 6

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Figure Caption

Fig. 6

Abnormal heart looping and gut formation in zebrafish Zic3 morphants. (A–C) Direction of cardiac looping (arrows) visualized by WISH of cardiac myosin light chain-2 (cmlc2) in 2 dpf embryos. Control embryos showed normal looping (A), whereas Zic3 morphants often showed mirror reversed (B) or unlooped (C) hearts. (D) Rescue of cardiac looping defects. Quantification of cardiac looping defects in control embryos is shown compared with embryos injected with Zic3 morpholino, Xenopus zic3 mRNA, or co-injected with Zic3 morpholino and Xenopus zic3 mRNA. (E–H) Analysis of gut looping by WISH with foxa3 in 2 dpf embryos. Control embryos (E) showed normal asymmetric positioning of the liver (L) and pancreas (P). Organ position (F), patterning (G), and number (H) were abnormal in Zic3 morphants. **p < 0.01 by Fisher′s exact test; statistical significance was determined through comparison of Zic3 MO + zic3 mRNA with Zic3 MO to demonstrate phenotypic rescue.

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Acknowledgments

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Reprinted from Developmental Biology, 364(1), Cast, A.E., Gao, C., Amack, J.D., and Ware, S.M., An essential and highly conserved role for Zic3 in left-right patterning, gastrulation and convergent extension morphogenesis, 22-31, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.