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Fig. 5 Chd7 deficiency affects vertebral mineralization.
Lateral view of control-MO (A) and chd7-MO-injected (B) fish at 3 dpf. Chd7 morphants exhibited a slight curvature of the long body axis (B). A significant percentage of the chd7-MO-injected (2 ng/nl) fish had deformed body axis shape (C). Lateral views of chd7 morphants (lower panel) and control siblings (upper panel) at 8 dpf (D) and 14 dpf (E) showed a reduced bone mineralization. Vertebrae with reduced or no hemal or neural spines were also observed in the chd7-MO-injected fish (F; black arrow). h, hemal spine; n, neural spine. ***<0.001.
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