ZFIN Image: Tam et al., 2012, Fig. 2

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Fig. 2

Zebrafish Candidate-Based Genetic Screen Identifies Regulators of CNS Angiogenesis and Barriergenesis (A and B) Morpholino-mediated knockdown of BBB-enriched transcripts in Tg(kdrl:egfp) embryos. As described in Experimental Procedures, following a 4 ng morpholino injection, DAPI-positive brain parenchymal nuclei (Parenchymal DAPI; A: right panels) were visually isolated by computationally removing brain vasculature-derived DAPI signal from the DAPI channel. Results representative of at least three independent experiments are shown. Endothelial cells are green, DAPI is blue, rhodamine-dextran is red. MO, morpholino. (C) Quantification of DAPI leakage across the zebrafish BBB. n = 6 (control_MO), n > 6 (DR6_MO, TROY_MO, Spock2_MO, Adcyap1r1_MO, Tspn5_MO, and Tnfrsf1a_MO). Data are presented as the mean ± standard error of the mean (SEM) (^***p < 0.005; ^**p < 0.01; ^*p < 0.05). See also Figure S2.

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Acknowledgments

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Reprinted from Developmental Cell, 22(2), Tam, S.J., Richmond, D.L., Kaminker, J.S., Modrusan, Z., Martin-McNulty, B., Cao, T.C., Weimer, R.M., Carano, R.A., van Bruggen, N., and Watts, R.J., Death Receptors DR6 and TROY Regulate Brain Vascular Development, 403-417, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell