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Fig. 4
QODG inhibits angiogenesis in regenerative caudal fin of zebrafish at 7 days post amputation.
(A) Vehicle control: Tg(fli1:EGFP) zebrafish caudal fins were clipped at the mid-fin level, then the fish were allowed to recover in 0.1% DMSO for 7 days post amputation (dpa). (B–D) QODG-treated groups: Tg(fli1:EGFP) zebrafish were allowed to recover in various concentrations of QODG (20, 60, 180 μM) for 7 dpa. Orange arrow indicates the amputation site in caudal fin of adult zebrafish for all figures. (E) Quantitative comparison of the lengths of regenerative vessel and fin in the vehicle control group and QODG-treated groups. Data are expressed as mean ± SD from three independent experiments. *, the length of regenerative vascularized fin tissue in QODG-treated group compared with that in the vehicle control group; ***, P<0.001 vs. vehicle control. #, the length of regenerative nonvascularized fin tissue in QODG-treated group compared with that in the vehicle control group; ###, P<0.001 vs. vehicle control. (F) Quantitative comparison of the vessel densities of regenerative caudal fin in the vehicle control group and QODG-treated groups. Data are expressed as mean ± SD from three independent experiments. *, the vessel density of regenerative caudal fin in QODG-treated group compared with that in the vehicle control group; ***, P<0.001 vs. vehicle control. Scale bars, 200 μm.