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Fig. S4
Assessment of ability of Drosophila Tfap2 to restore neural crest in tfap2a/c-deficient zebrafish embryos. (A,D,G) Dorsal views of a flat-mounted (A) wild-type zebrafish embryo or (D) an embryo injected with tfap2a and tfap2c targeting MOs alone or (G) with Drosophila tfap2 mRNA, fixed at 11 hpf and processed to reveal foxd3 expression. White asterisks, premigratory neural crest. Red asterisks, non-neural crest-derived tailbud. (B,E,H) Lateral view of (B) wild-type zebrafish embryo or (E) an embryo injected with tfap2a and tfap2c targeting MOs alone or (H) with Drosophila tfap2 mRNA, fixed at 22 hpf and processed to reveal dlx2a expression. White asterisks, migratory neural crest. Yellow asterisks, brain. (C,F,I) Lateral views of live embryos at 48 hpf that are (C) wild-type or tfap2a mutants (F) injected with tfap2c MO alone or (I) in conjunction with Drosophila tfap2 mRNA. (J) Histograms summarizing neural crest rescue results in tfap2a/c-deficient embryos injected with Drosophila (Dm) tfap2 mRNA. Number of embryos (n) per group is displayed below bar. Scale bars: in A, 100 μm for A,D,G; in B, 50 μm for B,E,H; in C, 100 μm; in F, 100 μm for F,I.