IMAGE

Fig. S2

ID
ZDB-IMAGE-120216-106
Source
Figures for Van Otterloo et al., 2012
Image
Figure Caption

Fig. S2

Additional neural crest derivatives rescued by tfap2 homologs. (A-F) All embryos are viewed dorsally and oriented with anterior to the left. (A) Uninjected embryos show clear foxd3 expression at 28 hpf marking the cranial ganglia (arrowheads, 23/23). (B) This expression is absent in embryos injected with MOs targeting tfap2a and tfap2c (0/24). Embryos injected with these MOs and subsequently injected with mRNA encoding (C) amphioxus tfap2 (29/35), (D) lamprey tfap2 (26/26), (E) zebrafish tfap2b (31/33) and (F) tfap2e (22/22) show restored foxd3 expression within the cranial ganglia on either one or both sides (mRNA was injected mosaically). (G) Graph showing cell counts of tlxa-positive cells at 22 hpf, a marker of Rohon-Beard and dorsal root ganglia sensory neuron precursor cells, found in the trunk of embryos injected similarly to those shown in A-F (n=10/group, *P<0.05 relative to tfap2a/tfap2c MO alone group). Of note, the incomplete absence of tlxa-positive cells in tfap2a/tfap2c MO-injected embryos is a previously described Tfap2-independent population (Li and Cornell, 2007).

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development