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Fig. S4
Inhibition of PKC affects tooth formation. Similarly to has (PKC) mutant, one tooth-phenotype was also observed in fish treated with PKC inhibitor. PMA – activator of PKC does not stimulate formation of additional teeth in WT, nor rescues tooth phenotype in the dak homozygote mutant. Cartilaginous skeletons were stained with Alcian blue at 6 dpf. Pharyngeal arches were dissected out and flat mounted.
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