Fig. S1
- ID
- ZDB-IMAGE-111223-16
- Genes
- Publication
- Blum et al., 2012 - Retinoic acid signaling controls the formation, proliferation and survival of the blastema during adult zebrafish fin regeneration
- All Figures
- Figures for Blum et al., 2012
Fig. S1 The hsp70:cyp26a1 line efficiently inhibits RA signaling. (A) Heat shock of hsp70:cyp26a1 embryos for 1 hour starting at 6 hpf results in close proximity of the first somite to the otic vesicle and a kink at the head-trunk boundary at 24 hpf, representative of complete loss of RA signaling. (B) Whole-mount in situ hybridization for egr2a, a marker of hindbrain rhombomeres 3 and 5, illustrates absence of rhombomere 5 in hsp70:cyp26a1 embryos, caused by posterior expansion of more anterior rhombomeres. hs, heat shock; o, otic vesicle; s, first somite; 3, rhombomere 3; 5, rhombomere 5. Scale bars: 100 μm.