Fig. 4 Depletion of sec61al1 does not affect expression of Nodal pathway genes in the left lateral plate mesoderm and epithalamus. (A) southpaw (spaw) is expressed in the left lateral plate mesoderm at 22 hpf in non-injected controls (NIC) (A′) as well as in sec61al1 morphants injected with 500 pgsec61al1ATG-MO (morphant: 15/16 left-side, 1/16 bilateral versus NIC: 9/11 left-side, 2/11 bilateral). (B) lefty1 expression is confined to the left side of the epithalamus at 25 hpf in NIC and (B′) sec61al1 morphants (NIC: 19/22 left-sided, 3/22 absent; morphant: 27/29 left-sided, 2/29 absent). All views are dorsal. Scale bar = 10 μm.
Reprinted from Developmental Biology, 360(1), Doll, C.A., Burkart, J.T., Hope, K.D., Halpern, M.E., and Gamse, J.T., Subnuclear development of the zebrafish habenular nuclei requires ER translocon function, 44-57, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.