Image
|
Figure Caption
Fig. 4 BAC modification process. (A) The UCSC genome browser showing two BACs (red arrows) spanning the gene nkx3.2 in zebrafish. (B) BAC modification by homologous recombination to insert a reporter gene and a drug selection cassette next to the translation start site of the gene (ATG). (C) A zebrafish carrying the modified BAC for the gene nkx3.2 expressing EGFP in sclerotomes (white arrows) at 24 hpf. Abbreviations: EGFP, enhanced green fluorescent protein; NEO, neomycin; LHA, left homology arm; RHA, right homology arm; TGA, stop codon.
Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ BMC Dev. Biol.