Fig. 1

Fig. 1 Effect of GH on hair cell bundle density.

(A) Phalloidin-labeled saccular epithelia of baseline, buffer-injected, and GH-injected zebrafish at post-sound exposure day 2 (psed2). The upper image shows the five locations of hair cell counts along the rostral-caudal axis of the saccule. The enlarged images to the right of the saccules are representative 100X images of saccules at 75% along the rostral-caudal axis. Scale bar = 100 μm, D = dorsal, R = rostral, * = presumed newly formed hair cell bundles, ∧ = scar formation characteristic of hair cell loss. (B) Mean (±S.E.) number of hair cell bundles/900 μm² at specified locations along the rostral-caudal axis of the saccule of baseline and buffer- or GH-injected zebrafish. N = 6; * P<0.001.