Fig. 4
Opsin trafficking is unaffected in rep1 mutants. Retinal cryosections were stained with markers for rod and cone photoreceptors in wild type (A–D) and rep1 mutants (E–H). The 1D1 marker labels rhodopsin (Rho), whereas antibodies are against blue opsin (BOPS) and green opsin (GOPS) in the photoreceptor outer segments of wild-type and rep1 mutants. The zpr-1 antibody labels the red/green double cones and reveals the disheveled morphology of the photoreceptors. All sections were counterstained with DAPI to visualize nuclei. Pyknotic nuclei, a hallmark of cell death, are seen in rep1 mutants and indicated by arrows. (Scale bar, 20 μm.)