|
Fig. 1 Genomic location, expression, domain structure and subcellular localization of talpid3 and its encoded protein. (A) The chicken (Gallus gallus) ta3 locus is located in a region of chromosome 5 that shows conserved synteny with zebrafish (Danio rerio) chromosome 17, mouse (Mus musculus) chromosome 12 and human (Homo sapiens) chromosome 14 (based on UCSC genome browser). (B) Schematic representation of the chicken and zebrafish Talpid proteins showing the five coiled-coil domains in each (pink and green boxes, respectively) predicted using the program in http://groups.csail.mit.edu/cb/multicoil/cgi-bin/multicoil.cgi/cgi-bin/multicoil. Despite the low sequence identity (33%) between the two proteins, the number and distribution of coiled-coil domains are conserved. The location of the lesion associated with the chick ta3 mutant allele at residue 366 between the 1st and 2nd coiled-coil domains is indicated. (C,D) Distribution of ta3 mRNA revealed by in situ hybridization of early cleavage stage (C) or 2 dpf (D) embryos. Note the abundant maternally derived transcript in C. (E,F) GFP-tagged Talpid3 protein localizes to the basal body and the daughter centriole in cells of wild-type embryos (E). This localization is unaffected by mutation of igu, which encodes the basal body protein Dzip1 (F).