Fig. 3

Fig. 3 houdini mutants verified by F3 segregation show molecular linkage to chromosome 5.

(A) The pool of F2 mutants validated by F3 segregation (“Adult Mut Pool”), shows strong linkage to the z22250 and z14591 alleles on chromosome 5 of the mutant G₀ grandparent. Very little linkage to the G₀ mutant alleles is evident using a pool of unvalidated mutants selected only by F2 larval behavior (“Larval Mut Pool”), or control F2 larval siblings (“Larval Sib Pool”) with these same markers. (B) Individuals composing the validated adult F2 mutant pool. 11/12 individuals are homozygous for the mutant z22250 allele, while 8/12 individuals are homozygous for the mutant z14591 allele. Individual #8 is homozygous for the mutant z22250 and wildtype z14591 alleles, indicating both the maternal and paternal copies of the chromosome underwent meiotic recombination between the houdini mutation and z14591. (C) 12 representative individuals from the unvalidated larval F2 mutant pool. The F2 individuals raised or pooled all performed above the hypersensitivity threshold and furthermore were among the most responsive 15% of their clutch. 5/12 larvae contain wildtype z22250 and z14591 alleles (marked with X) and are likely not homozygous mutants.