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Fig. S2 Hh signaling is not sensitizised in ext2 mutants. Morpholino injection experiment (A-D). Lateral view of 3 dpf control (A, C) and ext2 mutants (B, D). In (C, D) 14 ng MO1-shha have been injected in the one-cell stage which has resulted in U-shaped somites in a portion of injected embryos (see result section). The shape of the somites is emphasized in the right panel (A-D). Cyclopamine treatment experiment (E-H). ptc1 expression in 38 hpf embryos subjected to 50 μM cyclopamine 32-38 hpf (E-H). Dorsal view (E, F) and dorsolateral view (G-H). WT embryos (E, G) and ext2 mutants (F-H).
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Full text @ BMC Dev. Biol.