Fig. 6
Genetic control and cell specificity of pancreatic dkk3b(dkk3) expression Comparison of dkk3b(dkk3) expression in wild type (A and B) mib (C and D), Zoep (E), nls (F) and smo (G) mutant embryos at 36hpf. Trunk-specific expression is missing in Zoep, nls and smo mutant embryos (arrows indicate corresponding positions). Double labelling of dkk3b(dkk3) mRNA (blue in H and I) and GFP protein (green in H2 and I2) in the beta- and alpha- cell reporter lines Tg[ins:GFP] (H2) and Tg[gcga:GFP] (I2) as determined by confocal microscopy. Dkk3b(dkk3) does not colocalize with transgenic GFP expression in beta or alpha cells. Asterisks indicate cells with a strong dkk3b(dkk3) expression. Scale bars correspond to 50 μm. All embryos are shown from dorsal with anterior to the left (A–G).
Reprinted from Gene expression patterns : GEP, 11(8), Untergasser, G., Martowicz, A., Hermann, M., Töchterle, S., and Meyer, D., Distinct expression patterns of dickkopf genes during late embryonic development of Danio rerio, 491-500, Copyright (2011) with permission from Elsevier. Full text @ Gene Expr. Patterns