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Fig. 4 dscam mRNA is expressed in CaPs and VaPs and wildtype transcripts were knocked down with SBMOs.
A. A projected confocal stack of dscam mRNA (red) and isl2a mRNA (a CaP/VaP motoneuron marker [41]) expression (green), reveals that at 24 hpf dscam is expressed in both CaP and VaP (*; CaP and VaP pair to left, single CaP to right) as well as other cells that are likely motoneurons. B. RT-PCR results confirming uninjected embryos have wildtype dscam mRNA (395 bp band) while MO-injected embryos have increased dscam pre-mRNA (490 bp band). Starred lanes show dscam transcripts are absent after injecting 8.4 ng of dscam SBMO. Scale bar = 20 μm.