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Fig. S6 Mnx1-deficient embryos have expanded expression of the arx alpha cell marker. (A-D) Whole-mount in situ hybridization for arx; fluorescent images of chromogenic in situ hybridizations. Ventral view of 24 hpf (A) uninjected control, (B) control MO-injected, (C) Mnx1 MO1-injected and (D) Mnx1 MO2-injected embryos. Anterior to left. (E) Mean (± s.d.) number of arx-expressing cells. *, P<0.001 (t-test, two-tailed distribution, Bonferroni correction), from a minimum of 11 embryos per group.
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