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Fig. 4 Expression of fgf20a in the hindbrain is restricted to rhombomere centres by the opposing actions of Hdac1 and Notch signalling. In situ hybridisation analysis of fgf20a expression in (A,C) wild-type sibling, (B) hdac1 mutant and (D) mind bomb mutant embryos at 27hpf (A-D). Dorsal views of hindbrain are shown in each panel. Wild-type sibling embryos (A,C) exhibit weak, narrow stripes of fgf20a expression in the hindbrain at 27 hpf that are localised to rhombomere centres (signals in rhombomeres 4, 5 and 6 are indicated by red arrowheads). fgf20a expression is extinguished in the hindbrain of hdac1 mutant embryos (B). By contrast, mind bomb mutant embryos exhibit increased and ectopic expression of fgf20a in a broad domain within the hindbrain that encompasses multiple adjacent rhombomeres (D).