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Fig. 7 Treatment of smo–/– embryos with SU5402 from 10 to 20S leads to a loss of anterior and posterior otic character. Ears of smo–/– and sibling zebrafish embryos treated with 10 μM SU5402 and DMSO, respectively, from 10 to 20S. (A,B) Anti-acetylated tubulin antibody stain marking the kinocilia of the first-forming hair cells (arrows). (C-J) FITC-phalloidin stain marking cell outlines and stereociliary bundles of the sensory hair cells. (C-F) Views of the whole vesicle. (G,H) Medial views showing the maculae. (I,J) Lateral views showing the cristae. (K-N) Inner ears of live embryos. Arrows indicate otoliths. cp, semicircular canal projection tissue. (O-T) In situ hybridisation to anterior and posterior otic markers. Dots delineate the otic vesicle in O and P. O,P,S,T: Dorsal views; anterior to left, medial to top. A-N,Q,R: Lateral views; anterior to left, dorsal to top. Scale bars: 50 μm.