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Fig. 7
wdr18 and integrin β1b act genetically in the regulation of laterality development.
(A) A control embryo under a light microscope. (B) The overall morphology of embryos injected with 5 ng of itgb1b morpholino is indistinguishable from controls. (C) The percentage of control and itgb1b morphant embryos with left-sided (L), right-sided (R), or bilateral (Bi) spaw expression. (D, E, F) The morphology of embryos injected with 1.5 ng itgb1b morpholino (D) or 1 ng wdr18 MO1 (E), and embryos injected with a combination of 1 ng wdr18 MO1 and 1.5 ng itgb1b morpholino (F) look relatively normal, except for a minor difference in body curvature (F). (G) sox17 staining in embryos injected with a combination of 1 ng wdr18 MO1 and 1.5 ng itgb1b morpholino indicates that DFC migration is affected. Dorsal view, animal pole to the top. (H) An example of bilateral spaw expression in the LPM of embryos injected with a combination of 1 ng wdr18 MO1 and 1.5 ng itgb1b morpholino. Dorsal view, anterior to the top. (I) The percentage of control embryos injected with 1.5 ng itgb1b morpholino, 1ng wdr18 MO1, or a combination of 1 ng wdr18 MO1 and 1.5 ng itgb1b morpholino showing left-sided (L), right-sided (R), or bilateral (Bi) spaw expression.