Fig. 7
The number of GABA receptor puncta is increased on celsr3 mutant ON-bipolar terminals.
A) ON-bipolar cells in cryosection were stained with anti-PKC antibodies and anti-GABAγ2. The PKC label fields were overlayed on the GABA signal, and the number of puncta was counted in each terminal rotated and visualized in 3D. Image shows merged z-stacks. Scale bars are 5 μm. B) The GABA puncta in the ON-bipolar terminals per μm3 for WT and celsr3 mutants (n = 60 terminals for each). Error bars are standard error. C) Mosaic 2 dpf larva containing WT cells in the eye (yellow dashed line) of a celsr3 mutant animal. Despite a large WT eye field transplant, this animal remained OKR negative. The tan dashed line outlines larva body.