ZFIN Image: Lou et al., 2011, Fig. 3

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Figure Caption

Fig. 3

Promotion of myocardial differentiation by gata5/smarcd3b in transplant experiments. (A,N) Schematics of experiments in which cells are transplanted to the host embryo margin (A) or animal pole (N) at sphere (Sp, 4 hpf) stage. (B-M) Transgenic myl7:EGFP donor cells from wild-type (E-G,K-M) or gata5/smarcd3b-overexpressing (B-D,H-J) donors were transplanted to the margin of wild-type host embryos. Rhodamine (red) indicates the lineage tracer in all donor-derived cells, EGFP indicates donor cells that have differentiated to form cardiomyocytes. (O-Z) Transplants were performed as above, with wild-type (R-T,X-Z) or gata5/smarcd3b-overexpressing (O-Q,U-W) donor cells placed in the animal pole of 4 hpf hosts. Embryos are 48 hpf, ventral (B-D) and lateral (E-Z) views with anterior towards the top.

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