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Fig. S4 Dynamic alterations in renal transcription factors in lib mutants follow trends similar to wild-type embryos in rostral and central domains. Gene expression patterns of transcription factors in nephron progenitors in wild-type embryos compared with lib mutant embryos at the 20, 22, 24, and 28 somite stage. Embryos are shown in lateral views with anterior to the left, and have been stained by whole-mount in situ hybridization to mark kidney expression (purple) and the somites with mhc (red). Black lines indicate areas of high gene expression, and numbers correspond to the adjacent somite position. The spatial distributions of hnf1a, hnf4a, and hnf1bb transcripts were unaltered between 20 and 28 somites (28 somite stage not shown). The distributions of etv5 and irx3b changed in wild-types and lib at the 28 and 24 somite stages, respectively.