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Fig. 1
Generation of Tg(zpc:G4VP16/UAS:nfsB-mCherry) transgenic line. A: Ovary-specific G4VP16 mRNA expression was confirmed in adult Tg(zpc:G4VP16) females by RT-PCR. B: Genomic PCR of transgene inserts, zpc:G4VP16 and UAS:nfsB-mCherry, confirming bitransgenic genotype. C: Schematic diagram demonstrating cis-activation of nfsB-mCherry expression by zpc:G4VP16 in Tg(zpc:G4VP16/UAS:nfsB-mCherry) transgenic line. D: nfsB-mCherry expression in Tg(zpc:G4VP16/UAS:nfsB-mCherry) transgenics was confirmed by the detection of mCherry in ovarian tissues (oocytes), and by thepresence of mCherry protein in spawned eggs.
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