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Fig. 3
Zebrafish cathepsin D purification by affinity chromatography.
Western blotting of CD purified from 4 dpf zebrafish homogenate after purification by pepstatinyl-agarose affinity chromatography. FT = flow-through; W1-3-7-10 = washing fractions; E1-2-3 = eluted fractions; R = fraction of residual proteins after elution. Fractions were desalted, concentrated, denatured in Laemmli buffer, resolved by SDS-PAGE and blotted onto nitrocellulose. The filter was incubated with polyclonal antibody against rat CD. The position of standard molecular weight proteins is indicated. The arrow points to 41 kDa zebrafish CD.
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