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Fig. S1
The B 32UTR reporter is expressed in germ cells. Synthetic reporter RNAs were transcribed in vitro and both the EGFP-nanos and mCherry-B reporter RNAs were microinjected into 1-cell stage embryos, which were then imaged at approximately 24 hpf. Top panels: EGFP and mCherry protein expression; insets show a higher magnification image of the germ cells; Bottom panel: image overlays of both EGFP and mCherry protein expression showing coincident expression; image intensity of the mCherry expression was increased to allow for visualisation of protein co-expression in yellow.
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