ZFIN Image: Wiszniak et al., 2011, Fig. 3

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Figure Caption

Fig. 3

The last 144 nt of the HuB 32UTR are necessary and sufficient for post-transcriptional regulation of HuB mRNA.

(A) Schematic representation of the four HuB 32UTR deletion reporter RNAs constructed, termed A, B, C and D. The 32UTR regions present in each of the reporters are indicated by black bars, and are shown relative to the full-length 32UTR of 444 nucleotides at the top. (B) Expression of EGFP and mCherry protein in embryos injected with the indicated deletion mutant reporters. 200 pg of each reporter was injected. All EGFP images were taken at 2000 ms, and all mCherry images were taken at 150 ms. Higher magnification images of mCherry expression in the germ cells are shown as insets.

Acknowledgments

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