Fig. s6

Fig. s6

Maternal mis function is not required for the activation of target genes by ectopic Wnt/Β-catenin and FGF signaling. Embryos were assayed for goosecoid expression at 70% epiboly (8 h.p.f.; A–F) or chordin expression at 30% epiboly (5 h.p.f.; G–J). Uninjected wild-type (A,G) and mis (B,H) show dorsally restricted expression of gsc and chd. Wild-type embryos injected with ß-catenin (C,I) or fgf8a (E) mRNA show expanded domains of dorsal gene expression. mis mutant embryos injected with ß-catenin (D,J) or fgf8a (F) mRNA also show ectopic expression of dorsal genes, although, as noted in Supp. Fig. 4, the overall levels of gsc expression can be reduced.