Fig. s4
RAR signaling regulates gene expression in the VR/OS and in POM. Lateral views of heads of 24- to 25-hpf embryos treated with DMSO or 10 μM AGN from 3s and hybridized with the indicated probes. (A and B) AGN treatment causes down-regulation of vax1, net1a, cyb5a, itgA5, dact1, nlz2, and dhrs3a and up-regulation of rdh10a in the VR/OS (arrowheads). Triangles point to expression of vax1 in the ventral forebrain (A) and nlz2 in the dorsal retina (B). (C) AGN treatment decreases foxC1a and nlz1 expression in both anterior-ventral (arrowheads) and anterior-dorsal (triangles) POM as well as twist1a expression in dorsal POM only (triangles).