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Fig. 4

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ZDB-IMAGE-110621-21
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Figures for Machuca-Tzili et al., 2011
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Fig. 4

Expression of alternatively spliced transcripts in mbnl2 morphants. (A) RT-PCR analysis using primers tnnt2F and tnnt2R3 (arrows) yields two main bands of 318 bp and 282 bp in control embryos (ā€˜Cā€™), whereas the 282 bp product is not detectable in the morphants (MO). Boxes depict the exon content of each product after sequencing. Exons 4 (green) and 6 (yellow) are alternatively spliced. (B) The normal tnnt2 alternative splicing pattern is shown at different developmental stages. RT-PCR analysis of control embryos at the 18-somite stage (1) and 24 hpf (2) shows the expression of a 318 bp product. 51 hpf (3), 76 hpf (4) and adult (5) controls express both isoforms, 318 bp and 282 bp. (C) RT-PCR analysis of clcn1. Primers clcn1-134F and clcn1-87R (arrows) produce two bands, one of 588 bp (including exon 18a) and one of 555 bp (excluding exon 18a), which are expressed at a ratio of 1:1.2 in control embryos, whereas, in the mbnl2 morphants, the 555 bp product is the predominant form, shifting the ratio to 1:1.98. Differences in exon content are shown in the boxes. Exon 18a (red) is alternatively spliced and encodes amino acids FTKLSPAGGVK.

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