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Fig. 4 Cleavage stage and epiboly defects in lzap morphants are rescued by co-injection of lzap mRNA. A–F: Co-injection of lzap mRNA with 5′ untranslated region (UTR) morpholino (MO) partially rescued developmental defects of lzap morphants at 6 and 24 hours postfertilization (hpf). G: Schematic showing embryo viability at indicated time points following injection of lzap MO with or without lzap RNA and in embryos without injection. Data represent means and standard errors derived from more than 180 embryos from three independent experiments. H: lzap morphants display proliferative, mitotic, and apoptotic defects that are partially rescued through co-injection of lzap mRNA. Embryos were analyzed at 6 hpf by immunohistochemical staining using antibodies to PCNA, p-Histone H3, and cleaved Caspase-3 as indicated (original magnification, ×40). I–L: Indices for proliferation (I), mitosis (J), and apoptosis (K) were determined by dividing the number of cells staining for PCNA, p-Histone H3, or cleaved Caspase-3, respectively, by the total number of cells. Means and standard error are presented. Data are from sections of 20 independently injected embryos. For relative cell size determination, cell borders were marked on captured hematoxylin stained images and relative area (L) calculated by ImageJ software. Values are normalized to control cells. NS, not significant.