ZFIN Image: Campos et al., 2011, Fig. 2

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Figure Caption

Fig. 2

SNAP-tag allows labelling of different cell structures in the zebrafish embryo. A,B: Mosaic labelling of the basolateral membrane with Lgl2-SNAP and TMR Star (laser power to image Lgl2-SNAP: 20%). B: Zoom of the boxed area in A. C: Mosaic labelling of early endosomal membranes with SNAP-Rab5 and BG-DAF; arrows point at the endosomal population labelled with SNAP-Rab5 (laser power to image SNAP-Rab5: 3%). D: Mosaic labelling of early endosomal membranes with Rab5-CFP. A?D: 36-hpf embryos; projection of 5 planes of the embryo spinal cord; dotted lines delimit the embryo′s spinal cord; anterior to the left; scale bars = 10 μm.

Acknowledgments

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