Fig. 6

Fig. 6 hmgb genes are expressed in more restricted and gene-specific patterns during late embryogenesis. A selection of representative WISHs (A, D, F, G, M and N: 40× magnification) and transverse sections (B, C and E: 200× magnification, H–L, O–Q: 130× magnification) are shown. (A–E) 24 hpf: hmgb1a/1b and hmgb3a are mainly expressed in the rostral CNS (A–C) but also visible in the spinal cord (SC), in the lateral line primordium and in the intermediate cell mass (A, blue arrow and arrowhead, respectively). Only hmgb3a is under-represented in eyes (C). hmgb2a/2b are more highly and broadly expressed (D and E), with the notable exception of the somitic mesoderm (E, grey arrow). A moderately stained embryo is shown in panel D. (F–Q) 2 dpf: hmgb3b staining is almost undetectable, while all other hmgb signals are highly visible in the rostral part of the embryo, including the rostral CNS (F–H, M–O). hmgb1a and hmgb2a/2b probes strongly mark lateral line neuromasts (arrowheads in F, G and L) and endodermal derivates including gut and liver (arrows in F, G, I and L) while hmgb1b transcripts are detectable in the pronephric ducts (light blue arrow in M). hmgb3a remains faintly expressed in the eyes (N and O) and does not show staining in the gut nor in the lateral line neuromasts (P and Q). SC, spinal cord; Di, diencephalon; E, eye; R, retina; Le, lens.