ZFIN Image: Osborn et al., 2011, Fig. S5

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Figure Caption

Fig. S5 Myod RNA injection drives ectopic myogenesis and cdkn1c up-regulation.

Embryos injected with RNA encoding Myod were analysed at 15 ss immunohistochemically for MyHC (A,C) and by mRNA in situ hybridization for myog (B), prdm1 (C) and cdkn1c (D). Dorsal flatmounts, anterior to top. Bar: 100 μm. (A). Unilateral ectopic muscle in somitic and head mesodermal regions. (B). Myod represses myog mRNA in somites, probably due to premature differentiation, and induces ectopic myog mRNA the head region. (C). Ectopic myogenesis is not accompanied by ectopic prdm1 expression. (D). Two examples of diffuse low level ectopic cdkn1c mRNA up-regulation (brackets) after myod RNA injection.

Acknowledgments

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Reprinted from Developmental Biology, 350(2), Osborn, D.P., Li, K., Hinits, Y., and Hughes, S.M., Cdkn1c drives muscle differentiation through a positive feedback loop with Myod, 464-475, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.