IMAGE

Fig. 5

ID
ZDB-IMAGE-110214-59
Antibodies
Source
Figures for Maurya et al., 2011
Image
Figure Caption

Fig. 5 Modulation of Smad accumulation and Eng expression by Hh and BMP pathway activity. (A-A′) Parasagittal optical section and transverse sections at different levels along the rostrocaudal axis of ptc1hu1602;ptc2tj222 double homozygous mutant embryo at 20 ss and 24 hours, respectively, showing vastly expanded MPs (revealed by 4D9, red) and reciprocal loss of pSmad accumulation (green) compared with wild-type embryo at a similar stage (B,B′). Note expansion in domain of pSmad accumulation dorsally and ventrally in ptc mutants, especially at more caudal levels; compare with 24 hour wild-type embryo (B′). (C,D) Parasagittal optical sections of Tg(actin:GAL4);Tg(eng2a:eGFP)i233; disp1tf18bmutant embryo injected with UAS:dnPKA;tRFP at one- to two-cell stage. Most tRFP expressing fibres are also eGFP+ve, consistent with an autonomous effect of Hh pathway activity on eng2a induction. Note that not all fibres expressing tRFP (C) ectopically activated eng2a:eGFP (*); conversely, one eGFP+ve fibre (marked by an arrow in D) was tRFP–ve; this possibly represents a fibre induced by residual Hh signalling activity, as is sometimes observed in disp1tf18bmutant embryos. (E,F) Merged images of 24 hours Tg(eng2a:eGFP)i233 embryos expressing caALK3 under actin:GAL4 control in which the Hh pathway has been activated by injection of dnPKA mRNA or of UAS:Shh DNA. (E) dnPKA injection results in the ectopic activation of the eng2a:eGFP transgene (green) but this is suppressed in cells expressing caALK3 (red). Similarly, (F) late expression of Shh activates the eng2a:eGFP transgene in fast twitch fibres but this is suppressed by caAlk3 (red). On average, only 1.4 out of 13 tRFP+ve fibres also expressed eGFP (n=22 hemisegments from seven embryos).

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development