ZFIN Image: Tittle et al., 2011, Fig. 7

Image

Figure Caption

Fig. 7 Lens fibers express markers of terminal differentiation in uhrf1 and dnmt1 mutants. (A–I) Cryosections from 5 dpf embryos immunostained for the lens fiber markers Crystallin AlphaA (A–C, red), Lengsin (D–F, red), or Aquaporin 0 (G–I, red). In all sections, nuclei are counterstained with Sytox-Green (green). uhrf1 (B,E,H) and dnmt1 (C,F,I) fibers express these markers, indicating that the disorganized secondary fibers in the mutant lenses still express markers of terminal differentiation. Yellow arrows in panels C, E, and F indicate regions of the mutant cornea which ectopically express lens fiber genes. In all panels, mild mutants were selected for analysis. Anterior is to the right in all panels. Scale bars are 80 μm.

Figure Data

Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Biology, 350(1), Tittle, R.K., Sze, R., Ng, A., Nuckels, R.J., Swartz, M.E., Anderson, R.M., Bosch, J., Stainier, D.Y., Eberhart, J.K., and Gross, J.M., Uhrf1 and Dnmt1 are required for development and maintenance of the zebrafish lens, 50-63, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.